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Product Name
Recombinant PPR35 protein (His tag)
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Description
Recombinant Human PPP1R35 (Protein phosphatase 1 regulatory subunit 35) Fragment (aa 78-253) is a high-purity, stable protein designed for structural and functional studies. This fragment represents a soluble domain ideal for investigating the protein's role in centriole elongation and centrosome maturation during cell division.
Protein phosphatase 1 regulatory subunit 35 (PPP1R35) is a human protein critical for centriole duplication and centrosome function. During centriole duplication, it facilitates centriole elongation by recruiting the microtubule-binding elongation machinery through interaction with RTTN, enabling centriole-to-centrosome conversion. It may also contribute to primary cilia assembly. PPP1R35 localizes to the centrosome and centriole, specifically accumulating in the proximal centriolar lumen above the cartwheel early in duplication, where it co-localizes with RTTN. These functions underscore its importance in cell division and ciliogenesis, making it a key target for cell biology and cancer research.
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Protein name
Protein phosphatase 1 regulatory subunit 35
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Uniprot ID
Q8TAP8
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Gene Name
PPP1R35; C7orf47
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 78-253 aa (Q8TAP8) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 21.41 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 22 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"