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PPP1R15B antibody

Cat.#: 114153

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Special Price 239.0 USD

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Product Information

  • Product Name
    PPP1R15B antibody
  • Documents
  • Description
    PPP1R15B Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human brain tissue, human kidney tissue, human lung tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in SGC-7901 cells, HEK-293 cells, MCF7 cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 100-110 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CREP antibody; FLJ14744 antibody; PPP1R15B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PPP1R15B recombinant protein (Accession Number: NM_032833). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:1000

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:1000

    IP Result of anti-PPP1R15B;CReP (IP:Catalog No:114153, 3ug; Detection:Catalog No:114153 1:1000) with MCF-7 cells lysate 2500ug.

    IP Result of anti-PPP1R15B;CReP (IP:Catalog No:114153, 3ug; Detection:Catalog No:114153 1:1000) with MCF-7 cells lysate 2500ug.

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:114153(PPP1R15B;CReP antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:114153(PPP1R15B;CReP Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG .

    Immunofluorescent analysis of HepG2 cells using Catalog No:114153(PPP1R15B;CReP Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG .

  • Background
    Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosporylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic.
  • References
    • So JS, Cho S, Min SH, Kimball SR, Lee AH. IRE1α-Dependent Decay of CReP/Ppp1r15b mRNA Increases Eukaryotic Initiation Factor 2α Phosphorylation and Suppresses Protein Synthesis. Molecular and cellular biology. 35(16):2761-70. 2015.
    • Loveless TB, Topacio BR, Vashisht AA. DNA Damage Regulates Translation through β-TRCP Targeting of CReP. PLoS genetics. 11(6):e1005292. 2015.
    • Kernohan KD, Tétreault M, Liwak-Muir U. Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability. Human molecular genetics. 24(22):6293-300. 2015.
    • Friend K, Brooks HA, Propson NE, Thomson JA, Kimble J. Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation. PloS one. 10(9):e0139076. 2015.
    • Young SK, Willy JA, Wu C, Sachs MS, Wek RC. Ribosome Reinitiation Directs Gene-specific Translation and Regulates the Integrated Stress Response. The Journal of biological chemistry. 290(47):28257-71. 2015.
    • De Gassart A, Bujisic B, Zaffalon L. An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response. Proceedings of the National Academy of Sciences of the United States of America. 113(2):E117-26. 2016.
    • Kloft N, Neukirch C, von Hoven G. A subunit of eukaryotic translation initiation factor 2α-phosphatase (CreP/PPP1R15B) regulates membrane traffic. The Journal of biological chemistry. 287(42):35299-317. 2012.
    • Hahn CK, Lowrey CH. Eukaryotic initiation factor 2α phosphorylation mediates fetal hemoglobin induction through a post-transcriptional mechanism. Blood. 122(4):477-85. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"