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  • POLG2 antibody
  • POLG2 antibody
  • POLG2 antibody
  • POLG2 antibody

POLG2 antibody

Cat.#: 114002

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Product Information

  • Product Name
    POLG2 antibody
  • Documents
  • Description
    POLG2 Rabbit Polyclonal antibody. Positive IF detected in COLO 320 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in COLO 320 cells, HeLa cells, mouse colon tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, IHC, IF, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HP55 antibody; MTPOLB antibody; p55 antibody; PEOA4 antibody; POLB antibody; POLG BETA antibody; POLG2 antibody; POLGB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of POLG2 recombinant protein (Accession Number: BC009194). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114002(POLG2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114002(POLG2 antibody) at dilution of 1:100 (under 10x lens)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:1000

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:800

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:800

    Immunofluorescent analysis of COLO 320 cells using Catalog No:114002(POLG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of COLO 320 cells using Catalog No:114002(POLG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities. Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) [MIM:610131].Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"