-
Product Name
POLG2 antibody
- Documents
-
Description
POLG2 Rabbit Polyclonal antibody. Positive IF detected in COLO 320 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in COLO 320 cells, HeLa cells, mouse colon tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 55 kDa
-
Tested applications
ELISA, IHC, IF, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
HP55 antibody; MTPOLB antibody; p55 antibody; PEOA4 antibody; POLB antibody; POLG BETA antibody; POLG2 antibody; POLGB antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of POLG2 recombinant protein (Accession Number: NM_007215). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114002(POLG2 antibody) at dilution of 1:100 (under 10x lens)
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:1000
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:800
Immunofluorescent analysis of COLO 320 cells using Catalog No:114002(POLG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities. Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) [MIM:610131].Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"