POLG2 Rabbit Polyclonal antibody. Positive IF detected in COLO 320 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in COLO 320 cells, HeLa cells, mouse colon tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 55 kDa
ELISA, IHC, IF, WB
Human,Mouse,Rat; other species not tested.
HP55 antibody; MTPOLB antibody; p55 antibody; PEOA4 antibody; POLB antibody; POLG BETA antibody; POLG2 antibody; POLGB antibody
This antibody was obtained by immunization of POLG2 recombinant protein (Accession Number: BC009194). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114002(POLG2 antibody) at dilution of 1:100 (under 10x lens)
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:1000
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114002(POLG2 Antibody) at dilution of 1:800
Immunofluorescent analysis of COLO 320 cells using Catalog No:114002(POLG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities. Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) [MIM:610131].Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.
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