Recombinant PNKP protein (His tag)

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Recombinant Human PNKP (144-521) Fragment - A highly purified, stable protein fragment of the bifunctional polynucleotide phosphatase/kinase, specifically engineered for structural and biochemical studies of DNA repair mechanisms. This 378-amino acid fragment maintains optimal solubility and stability while representing a key functional region of this essential DNA repair enzyme.

The full-length PNKP protein plays a critical role in DNA damage repair through both non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. This bifunctional enzyme possesses both polynucleotide 3'-phosphatase and polynucleotide 5'-hydroxyl-kinase activities, enabling it to process DNA termini by removing 3'-phosphates and phosphorylating 5'-hydroxyl groups, thereby preparing DNA ends for subsequent extension and ligation steps in repair processes. PNKP localizes to the nucleus and chromosomes, where it specifically accumulates at sites of double-strand breaks, highlighting its direct involvement in maintaining genomic integrity. The selected fragment (144-521) represents a computationally optimized region designed to provide researchers with a reliable tool for investigating the structural and functional properties of this crucial DNA repair enzyme.