Plexin B2 antibody

Cat.#: 113966

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Product Information

  • Product Name
    Plexin B2 antibody
  • Documents
  • Description
    Plexin B2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF7 cells, mouse liver tissue, mouse ovary tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 80kd,240kd
  • Tested applications
    ELISA, IF, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    dJ402G11.3 antibody; KIAA0315 antibody; MM1 antibody; Nbla00445 antibody; PLEXB2 antibody; Plexin B2 antibody; PLXNB2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Plexin B2 recombinant protein (Accession Number: BC004542). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113966(PLXNB2 antibody) at dilution of 1:800

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113966(PLXNB2 antibody) at dilution of 1:800

    IP Result of anti-PLXNB2 (IP:Catalog No:113966, 4ug; Detection:Catalog No:113966 1:1000) with mouse liver tissue lysate 4000ug.

    IP Result of anti-PLXNB2 (IP:Catalog No:113966, 4ug; Detection:Catalog No:113966 1:1000) with mouse liver tissue lysate 4000ug.

    Immunofluorescent analysis of HepG2 cells using Catalog No:113966(PLXNB2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:113966(PLXNB2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"