Recombinant PLCD4 protein (His tag)

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Recombinant human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (hPLCD4) fragment (amino acids 1-438), a key phospholipase C enzyme that hydrolyzes PIP2 to generate secondary messengers DAG and IP3. This fragment contains the complete PH domain, EF-hand motifs, and PI-PLC X-box domain, making it an ideal tool for studying calcium signaling, phospholipid metabolism, and domain-specific functions.

Human PLCD4 (UniProt Q9BRC7) is a phosphoinositide-specific phospholipase C enzyme that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce two critical second messengers: diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG activates protein kinase C signaling, while IP3 triggers calcium release from intracellular stores. This enzyme plays essential roles in sperm acrosome reaction during fertilization, cell growth regulation, and liver regeneration, and modulates the Erk signaling pathway to influence proliferation. The full-length protein localizes to multiple intracellular compartments including membranes, endoplasmic reticulum, nucleus, and cytoplasm, functioning as a peripheral membrane protein. The isoform 2 variant additionally acts as a non-receptor guanine nucleotide exchange factor for GNAI3.