PIR antibody

Cat.#: 113833

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Product Information

  • Product Name
    PIR antibody
  • Documents
  • Description
    PIR Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 35 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    PIR antibody; Pirin antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PIR recombinant protein (Accession Number: NM_003662). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113833(PIR antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113833(PIR antibody) at dilution of 1:500

    IP Result of anti-PIR (IP:Catalog No:113833, 4ug; Detection:Catalog No:113833 1:300) with HeLa cells lysate 1200ug.

    IP Result of anti-PIR (IP:Catalog No:113833, 4ug; Detection:Catalog No:113833 1:300) with HeLa cells lysate 1200ug.

  • Background
    Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"