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Product Name
Phospho-PERK-T982 pAb
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Description
Polyclonal antibody to Phospho-PERK-T982
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
PEK antibody; WRS antibody; PERK antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding T980 of human PERK.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - Phospho-PERK-T982 pAb
Western blot analysis of extracts of C6 cells, using Phospho-PERK-T982 pAb at 1:1000 dilution.C6 cells were treated by ATP(5 mM) at 30u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
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