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  • Phospho-BCAR1-Y165 pAb

Phospho-BCAR1-Y165 pAb

Cat.#: 166529

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Product Information

  • Product Name
    Phospho-BCAR1-Y165 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-BCAR1-Y165
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    BCAR1 antibody; CAS antibody; CAS1 antibody; CASS1 antibody; CRKAS antibody; P130Cas antibody; BCAR1, Cas family scaffolding protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding Y165 of human BCAR1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-BCAR1-Y165 pAb

    Western blot - Phospho-BCAR1-Y165 pAb

    Western blot analysis of extracts of PC-12 cells, using Phospho-BCAR1-Y165 antibody at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"