Polyclonal antibody to Phospho-BCAR1-Y165
Human, Mouse, Rat
BCAR1 antibody; CAS antibody; CAS1 antibody; CASS1 antibody; CRKAS antibody; P130Cas antibody; BCAR1, Cas family scaffolding protein antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y165 of human BCAR1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-BCAR1-Y165 pAb
Western blot analysis of extracts of PC-12 cells, using Phospho-BCAR1-Y165 antibody at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
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