PHF5A Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 12-14 kDa
ELISA, WB, IP
Human, Mouse; other species not tested.
bK223H9.2 antibody; INI antibody; PHD finger protein 5A antibody; PHF5A antibody; SF3b14b antibody
This antibody was obtained by immunization of PHF5A recombinant protein (Accession Number: BC007321). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113814(PHF5A antibody) at dilution of 1:500
IP Result of anti-INI-1 (IP:Catalog No:113814, 3ug; Detection:Catalog No:113814 1:500) with HeLa cells lysate 4000ug.
The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.
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