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Product Name
PENK Polyclonal Antibody
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Description
Polyclonal antibody to PENK
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PENK antibody; PE antibody; PENK-A antibody; proenkephalin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human PENK (NP_001129162.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - PENK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PENK antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 20s.
Immunohistochemistry - PENK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using PENK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PENK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using PENK antibody at dilution of 1:100 (40x lens).
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Background
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"