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Product Name
PDE8A antibody
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Description
PDE8A Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 93kd,88kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ16150 antibody; HsT19550 antibody; PDE8A antibody; phosphodiesterase 8A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDE8A recombinant protein (Accession Number: NM_002605). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113670(PDE8A antibody) at dilution of 1:1000
IP Result of anti-PDE8A (IP:Catalog No:113670, 5ug; Detection:Catalog No:113670 1:1000) with mouse testis tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113670(PDE8A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113670(PDE8A antibody) at dilution of 1:100 (under 40x lens)
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Background
PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).
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