PARD3 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IP detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Positive IHC detected in human colon cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 180, 140-150, 100 kDa
ELISA, WB, IHC, FC, IP
Human,Mouse,Rat; other species not tested.
ASIP antibody; Baz antibody; Bazooka antibody; CTCL tumor antigen se2 5 antibody; FLJ21015 antibody; PAR 3 antibody; PAR3 antibody; PAR3 alpha antibody; PAR3A antibody; PAR3alpha antibody; PARD 3 antibody; PARD3 antibody; PARD3A antibody; SE2 5L16 antibody; SE2 5LT1 antibody; SE2 5T2 antibody
This antibody was obtained by immunization of PARD3 recombinant protein (Accession Number: BC011711). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113582(PARD3 antibody) at dilution of 1:300
IP Result of anti-PARD3 (IP:Catalog No:113582, 4ug; Detection:Catalog No:113582 1:200) with MCF-7 cells lysate 2900ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113582(PARD3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113582(PARD3 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug PARD3 antibody (Catalog No:113582, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
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