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Product Name
PARD3 antibody
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Description
PARD3 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IP detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Positive IHC detected in human colon cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 180, 140-150, 100 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASIP antibody; Baz antibody; Bazooka antibody; CTCL tumor antigen se2 5 antibody; FLJ21015 antibody; PAR 3 antibody; PAR3 antibody; PAR3 alpha antibody; PAR3A antibody; PAR3alpha antibody; PARD 3 antibody; PARD3 antibody; PARD3A antibody; SE2 5L16 antibody; SE2 5LT1 antibody; SE2 5T2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PARD3 recombinant protein (Accession Number: BC011711). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113582(PARD3 antibody) at dilution of 1:300
IP Result of anti-PARD3 (IP:Catalog No:113582, 4ug; Detection:Catalog No:113582 1:200) with MCF-7 cells lysate 2900ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113582(PARD3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113582(PARD3 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug PARD3 antibody (Catalog No:113582, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
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References
- Yu M, Yang S, Qiu Y. Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation. Journal of gastroenterology. 50(11):1103-13. 2015.
- Kaenel P, Antonijevic M, Richter S. Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway. International journal of oncology. 40(2):357-69. 2012.
- Kaenel P, Hahnewald S, Wotzkow C, Strange R, Andres AC. Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization. Development, growth & differentiation. 56(4):255-75. 2014.
- Song H, Bush RA, Vijayasarathy C, Fariss RN, Kjellstrom S, Sieving PA. Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis. Investigative ophthalmology & visual science. 55(4):2659-68. 2014.
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