Recombinant PAN3 protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Human PAN3 Fragment (aa 442-887) is a high-purity, stable protein fragment derived from the PAN2-PAN3 deadenylation complex subunit PAN3. This fragment, spanning residues 442 to 887, is ideal for structural studies, biochemical assays, and investigating mRNA deadenylation mechanisms, offering researchers a soluble and functional unit for exploring cytoplasmic mRNA turnover pathways.

The full-length PAN3 protein is a regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, a key cytoplasmic enzyme involved in mRNA turnover. It facilitates deadenylation by recruiting the catalytic subunit PAN2 to mRNA targets via interactions with poly(A)-binding protein (PABP) and GW182 proteins, playing critical roles in general and microRNA-mediated mRNA decay. PAN3 localizes to cytoplasmic P-bodies and exhibits isoform-specific functions: Isoform 1 stabilizes certain mRNAs (e.g., AU-rich element-containing transcripts) and influences mRNP remodeling, while Isoform 3 enhances deadenylation activity and promotes mRNA decay across multiple pathways, including nonsense-mediated decay. The protein's involvement in mRNA stability regulation impacts cell cycle progression and proliferation, underscoring its significance in gene expression control.