-
Product Name
Pan-PAX antibody
- Documents
-
Description
Pan-PAX Rabbit Polyclonal antibody. Positive IF detected in SKOV-3 cells. Positive IHC detected in human ovary tumor tissue, human renal cell carcinoma tissue. Positive IP detected in SKOV-3 cells. Positive WB detected in SKOV-3 cells. Observed molecular weight by Western-blot: 48 kDa; 56-60 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
paired box antibody; paired box 8 antibody; Paired box protein Pax antibody; Paired box protein Pax 8 antibody; Pan-PAX antibody; PAX 8 antibody; PAX8 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Pan-PAX recombinant protein (Accession Number: NM_003466). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113572(pan-PAX antibody) at dilution of 1:300
IP Result of anti-pan-PAX (IP:Catalog No:113572, 3ug; Detection:Catalog No:113572 1:800) with SKOV-3 cells lysate 2500ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113572(pan-PAX antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113572(pan-PAX antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed SKOV-3 cells using Catalog No:113572(pan-PAX Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.
-
References
- Tong GX, Memeo L, Colarossi C. PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract. The American journal of surgical pathology. 35(10):1473-83. 2011.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"