Recombinant PAK1 protein (His tag)

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Recombinant Human Serine/threonine-protein kinase PAK 1 (PAK1) fragment (residues 248-545) is a high-purity protein encompassing the complete kinase domain. This product is an essential tool for investigating cytoskeleton dynamics, cell signaling, and kinase activity in cancer, neurology, and metabolic research.

PAK1 is a pivotal protein kinase in human cells that regulates intracellular signaling downstream of integrins and receptor kinases. It plays critical roles in cytoskeleton reorganization, cell adhesion, migration, proliferation, apoptosis, and vesicle transport. PAK1 is activated by CDC42 and RAC1 GTPases and functions as a key effector linking them to the JNK MAP kinase pathway. It phosphorylates multiple substrates including RAF1, MAP2K1, MYL9, and BAD, influencing processes from insulin secretion to microtubule organization and synaptic stability. Subcellularly, PAK1 localizes to cytoplasm, focal adhesions, lamellipodia, membrane ruffles, invadopodia, and upon DNA damage, translocates to the nucleoplasm and chromatin. The provided fragment (248-545) contains the complete kinase domain, enabling focused study of PAK1's catalytic functions and signaling mechanisms.