PACT antibody

Cat.#: 113481

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Product Information

  • Product Name
    PACT antibody
  • Documents
  • Description
    PACT Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human skin cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, PC-12 cells. Observed molecular weight by Western-blot: 35 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DYT16 antibody; HSD 14 antibody; HSD14 antibody; PACT antibody; PKR assOCiated protein X antibody; PKR assOCiating protein X antibody; PRKRA antibody; RAX antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PACT recombinant protein (Accession Number: NM_003690). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113481(PRKRA antibody) at dilution of 1:800

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113481(PRKRA antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113481(PRKRA antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113481(PRKRA antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using PRKRA antibody Catalog No:113481 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using PRKRA antibody Catalog No:113481 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-PRKRA (IP:Catalog No:113481, 3ug; Detection:Catalog No:113481 1:700) with mouse testis tissue lysate 4000ug.

    IP Result of anti-PRKRA (IP:Catalog No:113481, 3ug; Detection:Catalog No:113481 1:700) with mouse testis tissue lysate 4000ug.

  • Background
    PRKRA represents protein kinase, interferon-inducible double stranded RNA dependent activator
  • References
    • Lugli G, Torvik VI, Larson J, Smalheiser NR. Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. Journal of neurochemistry. 106(2):650-61. 2008.
    • Li S, Zhu J, Fu H. Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA. Nucleic acids research. 40(2):884-91. 2012.
    • Schweitzer CJ, Jagadish T, Haverland N, Ciborowski P, Belshan M. Proteomic analysis of early HIV-1 nucleoprotein complexes. Journal of proteome research. 12(2):559-72. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"