CIGB-552 peptide

CIGB-552 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-HARIK-{d-Pro}-TFRR-{d-Leu}-KWKYKGKFW

Ac-His-Ala-Arg-Ile-Lys-D-Pro-Thr-Phe-Arg-Arg-D-Leu-Lys-Trp-Lys-Tyr-Lys-Gly-Lys-Phe-Trp

20

96.25%

C₁₃₁H₁₉₉N₃₉O₂₃

2689.23

1630763-23-3

Synthetic

Powder

It has been demonstrated that the peptide L-2—designed via alanine scanning of the Limulus-derived LALF32-51 region—represents a promising candidate for cellular-level intervention, with its cell-penetrating capacity serving as a functionally advantageous property. Through targeted modification of the primary structure of L-2, a second-generation peptide, CIGB-552, was developed. While its cytotoxic activity was evident, the underlying molecular mechanisms remained incompletely characterized. In this investigation, we observed that CIGB-552 elevates the expression of COMMD1, a protein implicated in copper homeostasis, sodium transport, and the NF-κB signaling cascade. Specifically, CIGB-552 promoted ubiquitination of RelA and suppressed NF-κB–mediated antiapoptotic activity, an effect that was abolished upon COMMD1 knockdown. Additionally, CIGB-552 reduced intrinsic antioxidant capacity and triggered peroxidation of proteins and lipids in susceptible cells. Collectively, these findings elucidate key aspects of the mode of action of CIGB-552, offering valuable insights for the development of future bio-active agents aimed at modulating cellular pathways.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lugo JM, Tafalla C, Oliva A, et al. Evidence for antimicrobial and anticancer activity of pituitary adenylate cyclase-activating polypeptide (PACAP) from North African catfish (Clarias gariepinus): Its potential use as novel therapeutic agent in fish and humans. Fish Shellfish Immunol. 2019;86:559-570. doi:10.1016/j.fsi.2018.11.056
• Fernu00e1ndez Massu00f3 JR, Oliva Argu00fcelles B, Tejeda Y, et al. The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013;2013:251398. doi:10.1155/2013/251398

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.