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Product Name
PABPC5 Polyclonal Antibody
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Description
Polyclonal antibody to PABPC5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
PABPC5 antibody; PABP5 antibody; polyadenylate-binding protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PABPC5 (NP_543022.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:4000
IHC 1:50 - 1:200 -
Validations
Western blot - PABPC5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PABPC5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"