PABPC1,PABP Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue, human testis tissue. Positive IF detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Positive WB detected in mouse testis tissue, HeLa cells, PC-3 cells, rat testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 71kd
ELISA, WB, IHC, IF, FC, IP
Human,Mouse,Rat; other species not tested.
PAB1 antibody; PABP antibody; PABP 1 antibody; PABP1 antibody; PABPC1 antibody; PABPC2 antibody; PABPL1 antibody; Poly(A) binding protein 1 antibody
This antibody was obtained by immunization of PABPC1,PABP recombinant protein (Accession Number: BC015958). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113474(PABPC1,PABP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:113474(PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody Catalog No:113474 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PABPC1,PABP (IP:Catalog No:113474, 4ug; Detection:Catalog No:113474 1:700) with mouse testis tissue lysate 4000ug.
1X10^6 MCF-7 cells were stained with 0.2ug PABPC1,PABP antibody (Catalog No:113474, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
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