PAAF1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HEK-293 cells, human brain tissue, Jurkat cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 30 kDa
ELISA, WB, IHC, IF
Human, Mouse; other species not tested.
FLJ11848 antibody; PAAF antibody; PAAF1 antibody; Protein G 16 antibody; Rpn14 antibody; WDR71 antibody
This antibody was obtained by immunization of PAAF1 recombinant protein (Accession Number: BC028628). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113473(PAAF1 antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.
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