P62/SQSTM1 antibody

Cat.#: 113556

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Product Information

  • Product Name
    P62/SQSTM1 antibody
  • Documents
  • Description
    P62/SQSTM1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human heart tissue. Positive WB detected in MCF7 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    P62 antibody; sequestosome 1 antibody; SQSTM1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_003900). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113556(SQSTM1 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113556(SQSTM1 antibody) at dilution of 1:500

    Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:113556(P62;SQSTM1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:113556(P62;SQSTM1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113556(SQSTM1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113556(SQSTM1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113556(SQSTM1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113556(SQSTM1 antibody) at dilution of 1:50 (under 10x lens)

  • Background
    SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"