P5 peptide

P5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-LQLQAEER-OH

H-Leu-Gln-Leu-Gln-Ala-Glu-Glu-Arg-OH

8

95.51%

C₄₁H₇₁N₁₃O₁₅

986.07

Synthetic

Powder

P5 (LQLQAEER, amino acids 53‐60 of FGF2) demonstrated strong binding activity (KD = 4.90 × 10^‐7 M) to FGFR2. P5 significantly inhibited the cell proliferation in FGFR2‐dependent manner in DU145 cells and retarded tumor growth in DU145 xenograft model with negligible toxicity toward normal organs. Further investigations found that the Gln4 and Glu6 residues of P5 bind to FGFR2 to abolish its activation. The cyclic derivative (DcP5) of P5 achieved reinforced stability and anticancer activity in vivo. P5 and its cyclic derivative DcP5 as potential candidates for anticancer therapy.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Zhang Y, Ouyang M, Wang H, Zhang B, Guang W, Liu R, Li X, Shih TC, Li Z, Cao J, Meng Q, Su Z, Ye J, Liu F, Hong A, Chen X. A cyclic peptide retards the proliferation of DU145 prostate cancer cells in vitro and in vivo through inhibition of FGFR2. MedComm (2020). 2020 Dec 14;1(3):362-375. doi: 10.1002/mco2.48. PMID: 34766128; PMCID: PMC8491194.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.