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OPTN antibody

Cat.#: 113415

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Product Information

  • Product Name
    OPTN antibody
  • Documents
  • Description
    OPTN Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human brain tissue. Positive IF detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, mouse brain tissue, mouse testis tissue. Observed molecular weight by Western-blot: 66 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    E3 14.7K interacting protein antibody; FIP 2 antibody; FIP2 antibody; GLC1E antibody; HIP 7 antibody; HIP7 antibody; Huntingtin yeast partner L antibody; HYPL antibody; NEMO related protein antibody; NRP antibody; optineurin antibody; OPTN antibody; TFIIIA INTP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of OPTN recombinant protein (Accession Number: NM_001008212). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113415(OPTN antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113415(OPTN antibody) at dilution of 1:800

    IP Result of anti-OPTN (IP:Catalog No:113415, 3ug; Detection:Catalog No:113415 1:800) with mouse brain tissue lysate 8000ug.

    IP Result of anti-OPTN (IP:Catalog No:113415, 3ug; Detection:Catalog No:113415 1:800) with mouse brain tissue lysate 8000ug.

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113415(OPTN antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113415(OPTN antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113415(OPTN antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113415(OPTN antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using OPTN antibody Catalog No:113415 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using OPTN antibody Catalog No:113415 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 MCF-7 cells were stained with 0.2ug OPTN antibody (Catalog No:113415, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug OPTN antibody (Catalog No:113415, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.
  • References
    • Slowicka K, Vereecke L, McGuire C. Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-κB signaling. European journal of immunology. 2015.
    • Osawa T, Mizuno Y, Fujita Y, Takatama M, Nakazato Y, Okamoto K. Optineurin in neurodegenerative diseases. Neuropathology : official journal of the Japanese Society of Neuropathology. 31(6):569-74. 2011.
    • Meng Q, Xiao Z, Yuan H. Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina. Molecular biology reports. 39(2):1119-24. 2012.
    • Wang C, Hosono K, Ohtsubo M. Interaction between optineurin and the bZIP transcription factor NRL. Cell biology international. 38(1):16-25. 2014.
    • Gao J, Ohtsubo M, Hotta Y, Minoshima S. Oligomerization of optineurin and its oxidative stress- or E50K mutation-driven covalent cross-linking: possible relationship with glaucoma pathology. PloS one. 9(7):e101206. 2014.
    • Tao Z, Wang H, Xia Q. Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity. Human molecular genetics. 24(9):2426-41. 2015.
    • Lazarou M, Sliter DA, Kane LA. The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature. 524(7565):309-14. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"