Odorranain-B1 peptide

Odorranain-B1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

AALKGCWTKSIPPKPCFGKR

Ala-Ala-Leu-Lys-Gly-Cys-Trp-Thr-Lys-Ser-Ile-Pro-Pro-Lys-Pro-Cys-Phe-Gly-Lys-Arg

20

97.7%

Synthetic

Powder

The peptide has been reported in different articles in different names. In addition to antimicrobial activity, the peptide also inhibits trypsin due to the disulfide-bonded trypsin inhibitory loop in the sequence (enzyme inhibitor) (Li J et al 2007 FASEB J 21: 2466-73). In this proteomic paper, 372 cDNA sequences of antimicrobial peptides were found in a single frog. Among them, 107 are new peptides. Approximately 40 peptides were synthesized and tested. Only 24 peptides with antimicrobial acitivities (APD entries 1291-1314) were registered into this database. OBR1 is active against E. coli ATCC25922 (MIC 5.83 ug/ml), S. aureus ATCC2592 (MIC 5.83 ug/ml), B. dysenteria (1.84 ug/ml), and C. albicans ATCC2002 (2.4 ug/ml). Derivatives: ORB-C (sequence CWTKSIPPKPC, disulfide bridged) has improved trypsin inhibition ability but lost its antimicrobial activity. ORB1 is N-terminal AA truncated form. Odorranain-B1 has Antibacterial, Antifungal activity. The source of Odorranain-B1 is Odorrana grahami, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li J, Xu X, Xu C, et al. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 2007;6(5):882-94.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.