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Product Name
NUP98 Monoclonal Antibody
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Description
Monoclonal antibody to NUP98
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Tested applications
WB, IP
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Species reactivity
Human
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Alternative names
NUP98 antibody; ADIR2 antibody; NUP196 antibody; NUP96 antibody; nucleoporin 98 antibody
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Isotype
Mouse IgG
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Preparation
Antigen: Recombinant protein of human NUP98
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - NUP98 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using NUP98 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.
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Background
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"