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Product Name
NTF2 antibody
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Description
NTF2 Mouse Monoclonal antibody. Positive WB detected in Hela cells, HepG2 cells, human heart tissue. Positive IF detected in MCF-7 cells, Hela cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 13kD
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
NTF 2 antibody; NTF2 antibody; nuclear transport factor 2 antibody; NUTF 2 antibody; NUTF2 antibody; Placental protein 15 antibody; PP15 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of NTF2 recombinant protein (Accession Number: NM_001322040). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107340(NUTF2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107340(NUTF2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107340(NUTF2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using NUTF2 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.
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