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Product Name
NR0B1 Polyclonal Antibody
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Description
Polyclonal antibody to NR0B1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
NR0B1 antibody; AHC antibody; AHCH antibody; AHX antibody; DAX-1 antibody; DAX1 antibody; DSS antibody; GTD antibody; HHG antibody; NROB1 antibody; SRXY2 antibody; nuclear receptor subfamily 0 group B member 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NR0B1 (NP_000466.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - NR0B1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NR0B1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - NR0B1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NR0B1 antibody . Blue: DAPI for nuclear staining.
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Background
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"