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Product Name
NOX1 Polyclonal Antibody
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Description
Polyclonal antibody to NOX1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NOX1 antibody; GP91-2 antibody; MOX1 antibody; NOH-1 antibody; NOH1 antibody; NADPH oxidase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NOX1 (NP_008983.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NOX1 Polyclonal Antibody
Western blot analysis of extracts of rat liver, using NOX1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NOX1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using NOX1 antibody at dilution of 1:100 (40x lens).
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Background
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"