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Product Name
NOMO1 antibody
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Description
NOMO1 Rabbit Polyclonal antibody. Positive WB detected in mouse colon tissue, A431 cells, COLO 320 cells, human brain tissue, mouse brain tissue. Positive IP detected in A431 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human colon tissue, human colon cancer tissue, human pancreas tissue. Observed molecular weight by Western-blot: 135 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NODAL modulator 1 antibody; Nomo antibody; NOMO1 antibody; PM5 antibody; pM5 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOMO1 recombinant protein (Accession Number: NM_014287). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:113300(NOMO1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:113300(NOMO1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:113300(NOMO1 Antibody) at dilution of 1:50
Immunofluorescent analysis of MCF-7 cells, using NOMO1 antibody Catalog No:113300 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NOMO1 (IP:Catalog No:113300, 4ug; Detection:Catalog No:113300 1:1000) with A431 cells lysate 1520ug.
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Background
NOMO1 represents NODAL modulator 1
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