Three letter code
Peptide Purity (HPLC)
Nkl71–100 is the peptide from aa residue 71 to 100 that comprises the membrane-lytic region of the turbot NK-lysin. Nkl71–100 exerts strong antiviral activity against spring viremia of carp virus (SVCV) by inhibiting not only the binding of viral particles to host cells, but also the fusion of virus and cell membranes, which requires a low pH context. Nkl71–100 could interfere in the replicative cycle of viruses whose entry into the host cell implies a process of receptor-mediated endocytosis, followed by another fusion step to the lysosome membrane at acidic pH. Nkl71–100 was able to induce the release of the vesicle content, which was significantly higher at pH 3, in a concentration-dependent manner. Nkl71–100 is at least (since potential inhibitory effects at late steps of infection cannot be discarded yet) affecting viral replication at an early stage in which virus-cell interactions are implicated, most probably at membrane levels. Nkl71–100 might still be playing an inhibitory role in other subsequent related mechanisms involving the same actors, such as the virus-cell membrane fusion.
Ideally Nkl71–100 should be stored in a freezer at or below -9C. Nkl71–100 should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
- Falco A, Medina-Gali RM, Poveda JA, Bello-Perez M, Novoa B, Encinar JA. Antiviral Activity of a Turbot (Scophthalmus maximus) NK-Lysin Peptide by Inhibition of Low-pH Virus-Induced Membrane Fusion. Mar Drugs. 2019;17(2)
About TFA salt
Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.
TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.
TFA Removal Service is recommended for:
- Peptides that will be used in cellular assays
- Peptides that will be used as APIs or in manufactured products
- For hydrophilic peptides containing numerous basic residues
- Analysed Sequence:H-EGVKSKLNIVCNEIGLLKSLCRKFVNSHIW-OH
- Chemical Formula:C154H255N43O41S2
- Sequence length:30
- Extinction coefficient:5690 M-1cm-1
- Mw average:3429.05
- Theoretical pI:9.71
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
Red: Hydrophobic uncharged residues, like F I L M V W A and P
Blue: Basic residues, like R K H and N-terminal -NH2
Green: Acidic residues, like D E and C-terminal -COOH
Black: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
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