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Product Name
NIN Polyclonal Antibody
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Description
Polyclonal antibody to NIN
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NIN antibody; SCKL7 antibody; ninein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - NIN Polyclonal Antibody
Western blot analysis of extracts of Jurkat cells, using NIN antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - NIN Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using NIN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NIN Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NIN Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NIN Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NIN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"