Nigrosin-OG2 peptide

Nigrosin-OG2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GLLGKILGVEKKVLCGLSGMC-OH

H-Gly-Leu-Leu-Gly-Lys-Ile-Leu-Gly-Val-Glu-Lys-Lys-Val-Leu-Cys-Gly-Leu-Ser-Gly-Met-Cys-OH

21

95.39%

C₉₃H₁₆₈N₂₄O₂₅S₃

2118.66

Synthetic

Powder

Nigrosin-OG2 is a peptide that has been studied for its antimicrobial and hemolytic activities. It is a member of the nigrocin-2 family of peptides, which are found in the skin secretions of the Yunnanfu frog (Rana grahami / Huia grahami).
Studies have shown that Nigrosin-OG2 exhibits reduced hemolytic activity against human erythrocytes compared to other peptides in the same family. This decrease in hemolytic activity is likely due to the replacement of polar residues on the hydrophilic face of the peptide's alpha-helix with neutral amino acids, resulting in a decrease in amphipathicity.
The primary structure of Nigrosin-OG2 has been determined and it has been found to be identical to two other peptides, grahamin 1 and grahamin 2, which were also isolated from R. grahami skin secretions.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li J, Xu X, Xu C, et al. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 2007;6(5):882-94.
• Conlon JM, Al-Ghaferi N, Abraham B, Jiansheng H, Cosette P, Leprince J, Jouenne T, Vaudry H. Antimicrobial peptides from diverse families isolated from the skin of the Asian frog, Rana grahami. Peptides. 2006 Sep;27(9):2111-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.