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Product Name
Nicastrin antibody
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Description
Nicastrin Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, C6 cells, human brain tissue. Positive IF detected in C6 cells. Observed molecular weight by Western-blot: 110kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
APH2 antibody; KIAA0253 antibody; NCSTN antibody; nicastrin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Nicastrin recombinant protein (Accession Number: NM_001290184). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113181(NCSTN antibody) at dilution of 1:800
Immunofluorescent analysis of C6 cells using Catalog No:113181(Nicastrin Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer’s disease patients (PMID: 12716934).
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