NFIC Polyclonal Antibody

Cat.#: 162425

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Product Information

  • Product Name
    NFIC Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NFIC
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    NFIC antibody; CTF antibody; CTF5 antibody; NF-I antibody; NFI antibody; nuclear factor I C antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFIC (NP_005588.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - NFIC Polyclonal Antibody

    Western blot - NFIC Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NFIC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

  • Background
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"