NEFM Polyclonal Antibody

Cat.#: 163181

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Product Information

  • Product Name
    NEFM Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NEFM
  • Tested applications
    WB, IF
  • Species reactivity
    Mouse, Rat
  • Alternative names
    NEFM antibody; NEF3 antibody; NF-M antibody; NFM antibody; neurofilament medium antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NEFM (NP_005373.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - NEFM Polyclonal Antibody

    Western blot - NEFM Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NEFM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunofluorescence - NEFM Polyclonal Antibody

    Immunofluorescence - NEFM Polyclonal Antibody

    Immunofluorescence analysis of mouse brain using NEFM antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - NEFM Polyclonal Antibody

    Immunofluorescence - NEFM Polyclonal Antibody

    Immunofluorescence analysis of rat brain using NEFM antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"