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Product Name
NEDD4L Polyclonal Antibody
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Description
Polyclonal antibody to NEDD4L
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NEDD4L antibody; NEDD4-2 antibody; NEDD4.2 antibody; PVNH7 antibody; RSP5 antibody; hNEDD4-2 antibody; E3 ubiquitin-protein ligase NEDD4-like antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NEDD4L Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NEDD4L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NEDD4L Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - NEDD4L Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody at dilution of 1:200 (40x lens).
Immunofluorescence - NEDD4L Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NEDD4L antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"