Human CD113/Nectin-3 (His Tag) recombinant protein

Human CD113/Nectin-3 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Nectin-3

PVRL3; CD113; DKFZP566B0846; PRR3; NECTIN-3; PVRR3; PPR3; CDW113; FLJ90624

Q9NQS3

PVRL3; PRR3

Human Cells

A DNA sequence encoding the extracellular domain of human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human PVRL3 comprises 354 amino acids and has a predicted molecular mass of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.
2. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.