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Product Name
NDUFV2 antibody
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Description
NDUFV2 Rabbit Polyclonal antibody. Positive IHC detected in human prostate tissue. Positive IF detected in Hela cells. Positive WB detected in mouse heart tissue, rat skeletal muscle tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 24 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NDUFV2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFV2 recombinant protein (Accession Number: NM_021074). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113087(NDUFV2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate using Catalog No:113087(NDUFV2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate using Catalog No:113087(NDUFV2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using NDUFV2 antibody Catalog No:113087 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NDUFV2 (IP:Catalog No:113087, 3ug; Detection:Catalog No:113087 1:400) with mouse heart tissue lysate 4000ug.
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Background
The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).
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References
- Chen T, Wu Q, Zhang Y, Zhang D. NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition. Brain research. 1625:102-10. 2015.
- Kim HL, Lee MY, Shin YJ. Increased Expression of Osteopontin in the Degenerating Striatum of Rats Treated with Mitochondrial Toxin 3-Nitropropionic Acid: A Light and Electron Microscopy Study. Acta histochemica et cytochemica. 48(5):135-43. 2015.
- Kumar V, Bouameur JE, Bär J. A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity. The Journal of cell biology. 211(5):1057-75. 2015.
- Miwa S, Jow H, Baty K. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nature communications. 5:3837. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"