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Product Name
NDUFA4L2 antibody
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Description
NDUFA4L2 Mouse Monoclonal antibody. Positive IF detected in MCF-7 cells, Hela cells. Positive WB detected in Hela cells. Observed molecular weight by Western-blot: 10kD
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ26118 antibody; NDUFA4L2 antibody; NUOMS antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of NDUFA4L2 recombinant protein (Accession Number: NM_001394960). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107325(NDUFA4L2 antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"