Nap-GFFKEH, Nap-1 peptide
Three letter code
Peptide Purity (HPLC)
Nap-1 is a noval class of histidine-containing hexapeptide derivatives used for in situ hydrogelation at the zinc ion-rich prostate tissue. Nap-1 displayed excellent self-assembly capability with a high sensitivity to zinc ions at about 0.1 equivalency. The molecular was rationally designed: (1) a dipeptide of phenylalanine (FF) which was utilized to promote molecular self-assembly and gelation; (2) a capping group of naphthalene to tune the hydrophobicity of Nap-1; (3) a terminus with glutamate (E) and histidine (H) to tune the hydrophilicity and to chelate zinc ions to form coordination bonds. Nap-1 was soluble in PBS and would form hydrogels at physiological pH (~pH 7.2) in the presence of zinc ions. The addition of 0.34 equiv. of EDTA destroyed the as-formed hydrogels of Nap-1 in 30 seconds, proving that metal chelations between zinc ions and peptides were vital for the self-assembly process.
Ideally Nap-GFFKEH, Nap-1 peptide should be stored in a freezer at or below -9C. Nap-GFFKEH, Nap-1 peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
- He S, Mei L, Wu C, et al. In situ hydrogelation of bicalutamide-peptide conjugates at prostate tissue for smart drug release based on pH and enzymatic activity. Nanoscale. 2019
About TFA salt
Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.
TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.
TFA Removal Service is recommended for:
- Peptides that will be used in cellular assays
- Peptides that will be used as APIs or in manufactured products
- For hydrophilic peptides containing numerous basic residues
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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