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Product Name
NAA60 Polyclonal Antibody
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Description
Polyclonal antibody to NAA60
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NAA60 antibody; HAT4 antibody; NAT15 antibody; NatF antibody; hNaa60 antibody; N-alpha-acetyltransferase 60 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human NAA60 (NP_079121.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NAA60 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NAA60 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - NAA60 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - NAA60 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - NAA60 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody at dilution of 1:200 (40x lens).
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Background
N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"