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Product Name
Myocilin antibody
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Description
Myocilin Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human stomach tissue, mouse skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GLC1A antibody; GPOA antibody; JOAG antibody; JOAG1 antibody; MYOC antibody; MyOCilin antibody; TIGR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of My℃ilin recombinant protein (Accession Number: NM_000261). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112989(MYoc antibody) at dilution of 1:300
IP Result of anti-MYoc (IP:Catalog No:112989, 4ug; Detection:Catalog No:112989 1:500) with mouse skeletal muscle tissue lysate 4000ug.
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Background
Myocilin (MYoc), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYoc are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYoc has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).
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