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Product Name
MYBPC3 Polyclonal Antibody
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Description
Polyclonal antibody to MYBPC3
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Tested applications
WB
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Species reactivity
Rat
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Alternative names
MYBPC3 antibody; CMD1MM antibody; CMH4 antibody; FHC antibody; LVNC10 antibody; MYBP-C antibody; myosin binding protein C, cardiac antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human MYBPC3 (NP_000247.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - MYBPC3 Polyclonal Antibody
Western blot analysis of extracts of rat heart, using MYBPC3 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"