MUC5AC motif peptide
Not For Human Use, Lab Use Only.
Cat.#: 319784
Special Price 146.80 USD
Availability:
1-2 weeks
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Product Name
MUC5AC motif peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-GTTPSPVPTTSTTSAP-OH
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Sequence
H-Gly-Thr-Thr-Pro-Ser-Pro-Val-Pro-Thr-Thr-Ser-Thr-Thr-Ser-Ala-Pro-OH
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Length (aa)
16
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Peptide Purity (HPLC)
95.37%
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Molecular Formula
C63H104N16O26
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Molecular Weight
1501.58
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CAS No.
244049-41-0
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PubChem CID
170910043
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Source
Synthetic
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Form
Powder
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Description
The MUC5AC motif peptide, with the sequence GTTPSPVPTTSTTSAP, is a 16-residue synthetic peptide derived from the human MUC5AC mucin gene. This peptide contains multiple potential O-glycosylation sites at serine and threonine residues, making it a suitable model for studying mucin-type O-glycosylation. The peptide's primary structure, particularly the presence of clustered hydroxy amino acids and proline-rich domains, influences its recognition by glycosyltransferases such as UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases (GaNTases).
In vitro studies have demonstrated that the MUC5AC motif peptide undergoes sequential O-glycosylation, with initial modifications by ubiquitous GaNTases like GaNTase-T1 and GaNTase-T2, followed by further glycosylation by specialized isoforms such as gpGaNTase-T7 and gpGaNTase-T9. These enzymes exhibit distinct substrate specificities, with gpGaNTase-T7 preferentially acting on di-GalNAc-substituted peptides, while gpGaNTase-T9 shows activity toward mono-GalNAc substrates. The peptide's O-glycosylation pattern is highly dependent on its primary sequence and the presence of prior GalNAc modifications, highlighting the hierarchical nature of mucin glycosylation.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Tetaert D, Ten Hagen KG, Richet C, Boersma A, Gagnon J, Degand P. Glycopeptide N-acetylgalactosaminyltransferase specificities for O-glycosylated sites on MUC5AC mucin motif peptides. Biochem J. 2001 Jul 1;357(Pt 1):313-20. doi: 10.1042/0264-6021:3570313. PMID: 11415465; PMCID: PMC1221957.
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About TFA salt
Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.
Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.
Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.
In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"