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Product Name
MTHFD1L antibody
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Description
MTHFD1L Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, COLO 320 cells, HeLa cells, mouse ovary tissue. Positive IP detected in HeLa cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 106kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ292B18.2 antibody; DKFZp586G1517 antibody; FLJ21145 antibody; FTHFSDC1 antibody; MTC1THFS antibody; MTHFD1L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTHFD1L recombinant protein (Accession Number: BC017477). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112777(MTHFD1L antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112777(MTHFD1L antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112777(MTHFD1L antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-MTHFD1L (IP:Catalog No:112777, 4ug; Detection:Catalog No:112777 1:1000) with HeLa cells lysate 2000ug.
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Background
MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.
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References
- Thomas A, Stevens AP, Klein MS. Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. Proteomics. 12(9):1437-51. 2012.
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