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Product Name
Mouse ITGA5&ITGB6 (Flag & His Tag) recombinant protein
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Description
Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.
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Uniprot ID
P43406
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Val988) of mouse ITGAV (NP_032428.2) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Pro708) of mouse ITGB6 (NP_067334.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse ITGAV&ITGB6 heterodimer was purified.
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Protein Species
Mouse
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Molecular weight
The recombinant heterodimer of mouse ITGAV&ITGB6 comprises 1728 (1000+728) amino acids and has a calculated molecular mass of 190.4 (110.7+79.7) KDa.
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Purity
> (32.4+62.5) % as determined by SDS-PAGE
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Validations
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