MOTS-c peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

MOTS-c is a mitochondrial-derived peptide encoded by a short open reading frame within the mitochondrial 12S ribosomal RNA gene. This 16-amino acid peptide is translated in the cytoplasm using the standard genetic code after its polyadenylated transcript is exported from the mitochondria. MOTS-c is highly conserved across species and is detectable in various tissues and in circulation.

The primary cellular action of MOTS-c involves targeting the folate-methionine cycle and its tethered de novo purine biosynthesis pathway. This interaction leads to the accumulation of the metabolic intermediate AICAR, which subsequently activates AMP-activated protein kinase. MOTS-c modulates cellular metabolism, including glucose utilization and fatty acid metabolism, with skeletal muscle appearing as a key target tissue. Its expression is regulated by metabolic state, such as fasting, and declines with age.

• Lee et al (2015) The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. 21(3) 443 PMID: 25738459
• Wei et al (2020) Mitochondrial-Derived Peptide MOTS-c Attenuates Vascular Calcification and Secondary Myocardial Remodeling via Adenosine Monophosphate-Activated Protein Kinase Signaling Pathway. Cardiorenal Med. 10(1) 42 PMID: 31694019
• Reynolds et al (2021) MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nat Commun. 12(1) 470 PMID: 33473109

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.