Human TPL2/MAP3K8/MEKK8 (GST Tag) recombinant protein

Human TPL2/MAP3K8/MEKK8 (GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Mitogen-activated protein kinase kinase kinase 8

TPL-2; MEKK8; C-COT; RP11-449I17.8; AURA2; MAP3K8; COT; ESTF; TPL2; COT/TPL2; FLJ10486; EST

P41279

MAP3K8; COT; ESTF

Baculovirus-Insect Cells

A DNA sequence encoding the human MAP3K8 isoform 1 (P41279-1) (Met 30-Arg 397) was fused with the GST tag at the N-terminus.

Human

The recombinant human MAP3K8/GST chimera consists of 592 amino acids and has a predicted molecular mass of 68 kDa as estimated in SDS-PAGE under reducing conditions.

> 91 % as determined by SDS-PAGE

Kinase activity untested