Recombinant MET14 protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Introducing the recombinant human METTL14 protein fragment (amino acids 116-403), a highly stable and soluble unit of the N(6)-adenosine-methyltransferase non-catalytic subunit. This fragment is ideal for structural studies, biochemical assays, and functional investigations into mRNA methylation mechanisms, offering researchers a reliable tool to explore epigenetic regulation.

The full-length METTL14 protein, encoded by the METTL14 gene in Homo sapiens, forms a heterodimer with METTL3 to create the core N6-methyltransferase complex responsible for methylating adenosine residues at the N(6) position in specific mRNAs. This methylation, occurring at 5'-[AG]GAC-3' consensus sites, plays a critical role in regulating mRNA stability, processing, and degradation. METTL14 serves as the RNA-binding scaffold within the complex, recognizing substrate mRNAs rather than catalyzing the methylation reaction itself. The protein is involved in key biological processes, including circadian rhythm regulation, embryonic stem cell differentiation, cortical neurogenesis, and spermatogenesis, by promoting the destabilization of target transcripts. METTL14 is localized to the nucleus, where it functions in mRNA modification pathways that influence cellular differentiation and development.